ARG56894

anti-TGFBI antibody

anti-TGFBI antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

publication_link 参考文献4

概述

产品描述 Rabbit Polyclonal antibody recognizes TGFBI
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 TGFBI
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 481-683 of Human TGFBI (NP_000349.1).
偶联标记 Un-conjugated
別名 CDGG1; LCD1; RGD-CAP; CSD2; CSD; Beta ig-h3; CSD1; Transforming growth factor-beta-induced protein ig-h3; RGD-containing collagen-associated protein; BIGH3; CDG2; CSD3; Kerato-epithelin; CDB1; EBMD

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Rat kidney, Mouse heart and BxPC3
实际分子量 ~ 74 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 21810 Mouse TGFBI

GeneID: 7045 Human TGFBI

Swiss-port # P82198 Mouse Transforming growth factor-beta-induced protein ig-h3

Swiss-port # Q15582 Human Transforming growth factor-beta-induced protein ig-h3

基因名称 TGFBI
全名 transforming growth factor, beta-induced, 68kDa
背景介绍 This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
生物功能 Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. [UniProt]
细胞定位 Secreted. Secreted, extracellular space, extracellular matrix. Note=May be associated both with microfibrils and with the cell surface (PubMed:8077289). [UniProt]
预测分子量 75 kDa
翻译后修饰 Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).

The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG56894 anti-TGF beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG56894 anti-TGF beta 1 antibody at 1:100 dilution.

  • ARG56894 anti-TGF beta 1 antibody WB image

    Western blot: 25 µg of Rat kidney, Mouse heart and BxPC-3 cell lysates stained with ARG56894 anti-TGF beta 1 antibody at 1:1000 dilution.

  • ARG56894 anti-TGF beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG56894 anti-TGF beta 1 antibody at 1:100 dilution.

文献引用

MicroRNA-411-3p inhibits bleomycin-induced skin fibrosis by regulating transforming growth factor-β/Smad ubiquitin regulatory factor-2 signalling

WB / Mouse

Ziyan Zhang et al.
J Cell Mol Med.,  (2021)

publication_link

 

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Transcriptomic analysis identifies upregulation of secreted phosphoprotein 1 in silicotic rats.

WB / Rat

Wenchen Cai et al.
Experimental and Therapeutic Medicine.,  (2021)

publication_link

 

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MiR-411-3p alleviates Silica-induced pulmonary fibrosis by regulating Smurf2/TGF-β signaling.

WB, IHC-P / Mouse

Gao Xuemin et al.
Exp Cell Res.,  (2020)

publication_link

 

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Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α

WB / Rat

Chen Ying-Ying et al.
Preprint.,  (2019)

publication_link

 

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