ARG56429

anti-TGF beta 1 antibody

anti-TGF beta 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

publication_link 参考文献7

概述

产品描述 Rabbit Polyclonal antibody recognizes TGF beta 1
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 This antibody detects endogenous levels of TGF beta 1 protein.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 TGF beta 1
抗原物种 Human
抗原 Synthetic peptide around aa. 341-390 of Human TGF beta 1.
偶联标记 Un-conjugated
別名 TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
纯度 > 95% (by SDS-PAGE).
缓冲液 PBS (pH 7.2) and 0.05% Sodium azide.
抗菌剂 0.05% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 21803 Mouse TGFB1

GeneID: 59086 Rat TGFB1

GeneID: 7040 Human TGFB1

基因名称 TGFB1
全名 transforming growth factor, beta 1
背景介绍 This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009]
生物功能 Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt]
预测分子量 44 kDa
翻译后修饰 Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

检测图片 (3) Click the Picture to Zoom In

  • ARG56429 anti-TGF beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG56429 anti-TGF beta 1 antibody.

  • ARG56429 anti-TGF beta 1 antibody WB image

    Western blot: 40 µg of 1) L02, 2) HEK293T, 3) AML-12, and 4) H9C2 whole cell lysates stained with ARG56429 anti-TGF beta 1 antibody at 1:500 dilution.

  • ARG56429 anti-TGF beta 1 antibody WB image

    Western blot: 1) HeLa, 2) SP2/0, 3) H9C2, and 4) MCF-7 cell lysates stained with ARG56429 anti-TGF beta 1 antibody at 1:500 dilution.

文献引用

Ozonated saline intradermal injection: promising therapy for accelerated cutaneous wound healing in diabetic rats

IHC-P / Rat

Ahmed Hesham et al.
Front Vet Sci.,  (2023)

publication_link

 

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LC-QToF chemical profiling of Euphorbia grantii Oliv. and its potential to inhibit LPS-induced lung inflammation in rats via the NF-κB, CY450P2E1, and P38 MAPK14 pathways

IHC-P / Rat

Mai Hussin Radi et al.
Inflammopharmacology.,  (2023)

publication_link

 

hr_line

Quercetin Alleviates Pulmonary Fibrosis in Silicotic Mice by Inhibiting Macrophage Transition and TGF-β-Smad2/3 Pathway

WB / Mouse

Fei Geng et al.
Curr. Issues Mol. Biol,  (2023)

publication_link

 

hr_line

Effect of Sex Differences in Silicotic Mice

IHC-P, WB / Mouse

Fuyu Jin et al.
Int J Mol Sci,  (2022)

publication_link

 

hr_line

Quercetin Alleviates Pulmonary Fibrosis in Mice Exposed to Silica by Inhibiting Macrophage Senescence

WB / Mouse

Fei Geng et al.
Front Pharmacol.,  (2022)

publication_link

 

hr_line

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis

WB / Rat

Tian Li et al.
Oxid Med Cell Longev.,  (2021)

publication_link

 

hr_line

Effect of hydrogen inhalation on IL-40 and SIgA in a Rat Model of Pulmonary Mucosal Immunity.

WB / Rat

Ma Yiping et al.
Preprint.,  (2020)

publication_link

 

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