ARG57594
anti-TFII-I antibody
anti-TFII-I antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TFII-I |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TFII-I |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human TFII-I. |
| 偶联标记 | Un-conjugated |
| 別名 | BTKAP1; IB291; TFII-I; WBS; General transcription factor II-I; SRF-Phox1-interacting protein; BAP135; BTK-associated protein 135; Bruton tyrosine kinase-associated protein 135; WBSCR6; BAP-135; Williams-Beuren syndrome chromosomal region 6 protein; GTFII-I; SPIN; DIWS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | BT-474 | ||||||
| 实际分子量 | 132 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GTF2I |
| 全名 | general transcription factor IIi |
| 背景介绍 | This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013] |
| 生物功能 | Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. [UniProt] |
| 预测分子量 | 112 kDa |
| 翻译后修饰 | Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation. Sumoylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG57594 anti-TFII-I antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophagus stained with ARG57594 anti-TFII-I antibody.
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ARG57594 anti-TFII-I antibody WB image
Western blot: 25 µg of BT-474 cell lysate stained with ARG57594 anti-TFII-I antibody at 1:500 dilution.
