ARG63248
anti-TFE3 antibody
anti-TFE3 antibody for Western blot and Human
Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes TFE3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TFE3 |
| 抗原物种 | Human |
| 抗原 | SHAAEPARDGVEAS-C |
| 偶联标记 | Un-conjugated |
| 別名 | bHLHe33; TFEA; Class E basic helix-loop-helix protein 33; Transcription factor E3; RCCX1; RCCP2 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.[provided by RefSeq, Dec 2009] |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Sumoylated; does not affect dimerization with MITF. |
检测图片 (1) Click the Picture to Zoom In
-
ARG63248 anti-TFE3 antibody WB image
Western Blot: HEK293 lysate (35 µg protein in RIPA buffer) stained with ARG63248 anti-TFE3 antibody at 1 µg/ml dilution.
