ARG66050

anti-TECK antibody (Biotin)

anti-TECK antibody (Biotin) for ELISA,Western blot and Human

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes TECK
反应物种 Hu
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 TECK
抗原物种 Human
抗原 E. coli derived recombinant Human TECK.
(QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL)
偶联标记 Biotin
別名 Chemokine TECK; Ckb15; SCYA25; Thymus-expressed chemokine; Small-inducible cytokine A25; TECK; C-C motif chemokine 25

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66049 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6370 Human CCL25

Swiss-port # O15444 Human C-C motif chemokine 25

基因名称 CCL25
全名 chemokine (C-C motif) ligand 25
背景介绍 This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
生物功能 Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt]
预测分子量 17 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG66050 anti-TECK antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human TECK stained with ARG66050 anti-TECK antibody (Biotin), under non-reducing conditions.

  • ARG66050 anti-TECK antibody (Biotin) standard curve image

    Direct ELISA: ARG66050 anti-TECK antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66050 anti-TECK antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human TECK stained with ARG66050 anti-TECK antibody (Biotin), under reducing conditions.

  • ARG66050 anti-TECK antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66050 anti-TECK antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66049 anti-TECK antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.