ARG40758
anti-TEAD1 antibody
anti-TEAD1 antibody for ChIP,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TEAD1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ChIP, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TEAD1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 135-215 of Human TEAD1 (NP_068780.2). |
| 偶联标记 | Un-conjugated |
| 別名 | AA; Transcription factor 13; NTEF-1; TEA domain family member 1; REF1; TEF-1; Protein GT-IIC; TCF13; Transcriptional enhancer factor TEF-1; TCF-13; TEAD-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse heart | ||||||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P28347 Human Transcriptional enhancer factor TEF-1 Swiss-port # P30051 Mouse Transcriptional enhancer factor TEF-1 |
|---|---|
| 基因名称 | TEAD1 |
| 全名 | TEA domain family member 1 (SV40 transcriptional enhancer factor) |
| 背景介绍 | This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010] |
| 生物功能 | Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 48 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40758 anti-TEAD1 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG40758 anti-TEAD1 antibody.
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ARG40758 anti-TEAD1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung stained with ARG40758 anti-TEAD1 antibody at 1:100 dilution.
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ARG40758 anti-TEAD1 antibody WB image
Western blot: 25 µg of Mouse heart lysate stained with ARG40758 anti-TEAD1 antibody at 1:1000 dilution.
