ARG52443
anti-TDP43 antibody
anti-TDP43 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TDP43 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TDP43 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of human TDP-43. |
| 偶联标记 | Un-conjugated |
| 別名 | TAR DNA-binding protein 43; TDP-43; ALS10 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~ 43kDa TDP-43 protein in Western blots of Rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | PBS and 30% Glycerol |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TARDBP |
| 全名 | TAR DNA binding protein |
| 背景介绍 | TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms. |
| 研究领域 | Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. |
检测图片 (1) Click the Picture to Zoom In
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ARG52443 anti-TDP43 antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~58k TR-α2 protein stained with ARG52443 anti-TDP43 antibody.
