ARG20999
anti-TCR beta antibody [H57-597] (PE-Cyanine 5)
anti-TCR beta antibody [H57-597] (PE-Cyanine 5) for Depletion,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Mouse
概述
| 产品描述 | PE-Cyanine 5-conjugated Hamster Monoclonal antibody [H57-597] recognizes TCR beta |
|---|---|
| 反应物种 | Ms |
| 应用 | Depletion, FACS, ICC/IF, IHC-P |
| 特异性 | Mouse TCRβ. The clone H57-597 does not react with γδ TCR-bearing cells. Plate-bound or soluble H57-597 activates αβ TCR-bearing T cells. In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. In vivo administration of H57-597 has been shown to deplete αβ TCR-bearing cells to near completion and prevent graft rejection. |
| 宿主 | Hamster |
| 克隆 | Monoclonal |
| 克隆号 | H57-597 |
| 同位型 | IgG2 |
| 靶点名称 | TCR beta |
| 抗原物种 | Mouse |
| 抗原 | TCR affinity purified from mouse T-cell hybridoma DO-11.10 |
| 偶联标记 | PE-Cyanine 5 |
| 別名 | TCRB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS, 0.1% Sodium azide and Sucrose. |
| 抗菌剂 | 0.1% Sodium azide |
| 稳定剂 | Sucrose |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TRB |
|---|---|
| 全名 | T cell receptor beta chain |
| 背景介绍 | T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas. [provided by RefSeq, Jul 2008] |
| 预测分子量 | 35 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG20999 anti-TCR beta antibody [H57-597] (PE-Cyanine 5) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20999 anti-TCR beta antibody [H57-597] (PE-Cyanine 5).
