ARG65529
anti-TCR alpha + TCR beta antibody [R73]
anti-TCR alpha + TCR beta antibody [R73] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Rat,Primates
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [R73] recognizes TCR alpha + TCR beta |
|---|---|
| 反应物种 | Rat, NHuPrm |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | R73 |
| 同位型 | IgG1 |
| 靶点名称 | TCR alpha + TCR beta |
| 抗原 | Rat T blasts and erythrocytes |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: In vitro activation and in vivo depletion of TCR alpha/beta T cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression._x000D_ |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | TCR alpha: 30 kDa TCR beta: 35 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65529 anti-TCR alpha + TCR beta antibody [R73] FACS image
Flow Cytometry: Rat thymocyte suspension stained with ARG65529 anti-TCR alpha + TCR beta antibody [R73] at 1.6 μg/ml dilution.
