ARG64126

anti-TCF3 / ITF1 antibody

anti-TCF3 / ITF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes TCF3 / ITF1
反应物种 Hu
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 TCF3 / ITF1
抗原物种 Human
抗原 C-KAPRARTSPDEDED
偶联标记 Un-conjugated
別名 TCF-3; E47; VDIR; Class B basic helix-loop-helix protein 21; Immunoglobulin transcription factor 1; Transcription factor 3; Kappa-E2-binding factor; bHLHb21; E2A; Transcription factor ITF-1; Transcription factor E2-alpha; ITF1; Immunoglobulin enhancer-binding factor E12/E47

应用说明

应用建议
应用 推荐稀释比
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 68 kDa

属性

形式 Liquid
纯化 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6929 Human TCF3

Swiss-port # P15923 Human Transcription factor E2-alpha

背景介绍 This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]
研究领域 Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
翻译后修饰 Phosphorylated following NGF stimulation.

检测图片 (2) Click the Picture to Zoom In

  • ARG64126 anti-TCF3 / ITF1 antibody WB image

    Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64126 anti-TCF3 antibody at 0.01 µg/ml dilution.

  • ARG64126 anti-TCF3 / ITF1 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64126 anti-TCF3 antibody at 3.8 µg/ml dilution followed by AP-staining.