ARG54636
anti-TBP / TATA Binding Protein antibody [1TBP18]
anti-TBP / TATA Binding Protein antibody [1TBP18] for Immunoprecipitation,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Gene Regulation antibody
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody [1TBP18] recognizes TBP / TATA Binding Protein |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 不反应物种 | Dm, Xenopus, Yeast |
| 应用 | IP, WB |
| 特异性 | This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1TBP18 |
| 同位型 | IgG1 |
| 靶点名称 | TBP / TATA Binding Protein |
| 抗原物种 | Human |
| 抗原 | Recombinant human TBP |
| 抗原表位 | 1-20 a.a. |
| 偶联标记 | Un-conjugated |
| 別名 | TATA-binding factor; SCA17; TATA sequence-binding protein; Transcription initiation factor TFIID TBP subunit; GTF2D1; TATA-box factor; TFIID; GTF2D; HDL4; TATA-box-binding protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G affinity chromatography |
| 缓冲液 | PBS (pH 7.4) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TBP |
| 全名 | TATA box binding protein |
| 背景介绍 | The TATA-binding protein (TBP) plays acentral role in the assembly of mosteukaryotic transcription initiation complexes.TBP assembles with other proteins to formunique multimeric complexes for each of thethree different nuclear RNA polymerases.TBP has been cloned from a variety ofspecies and consists of two distinct domains.The C-terminal domain (~180 amino acids)is highly conserved among species,whereas the N-terminal domain variesconsiderably in length and sequence amongdifferent species. The conserved C-terminaldomain contains the DNA-binding region aswell as regions that interact with positive andnegative regulatory proteins. In human TBP,the non-conserved N-terminal domainincludes a stretch of glutamine residuesranging from 26 to 42. Several researchgroups have found an association betweenexpansion of this polyglutamine tract withrare forms of spinocerebellar ataxia andother neurodegenerative diseases including Huntington's disease. |
| 生物功能 | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. [UniProt] |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 38 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54636 anti-TBP / TATA Binding Protein antibody [1TBP18] WB image
Western blot: 30 µg of Mouse testis lysate stained with ARG54636 anti-TBP / TATA Binding Protein antibody [1TBP18] at 1:500 dilution.
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ARG54636 anti-TBP / TATA Binding Protein antibody [1TBP18] WB image
Western blot: A. rhuTBP and HeLa cell nuclear extract; B. Epitope mapping: fragments of rhuTBP samples stained with ARG54636 anti-TBP / TATA Binding Protein antibody [1TBP18] at 2 µg/ml dilution.
客户反馈
Average
anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
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WB / Human
Rhodiola crenulata Suppresses High Glucose-Induced Matrix Metalloproteinase Expression and Inflammatory Responses by Inhibiting ROS-Related HMGB1-TLR4 Signaling in Endothelial Cells.
WB / Human
