ARG59077
anti-TAP2 antibody
anti-TAP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TAP2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TAP2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 430-680 of Human TAP2 (NP_000535.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Really interesting new gene 11 protein; Peptide transporter involved in antigen processing 2; D6S217E; ATP-binding cassette sub-family B member 3; Peptide transporter PSF2; Antigen peptide transporter 2; ABC18; ABCB3; APT2; RING11; PSF-2; Peptide transporter TAP2; PSF2; Peptide supply factor 2 |
应用说明
| 预测反应性说明 | Rat | ||||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse spleen | ||||||||
| 实际分子量 | 76kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TAP2 |
| 全名 | transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) |
| 背景介绍 | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014] |
| 生物功能 | Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=The transmembrane segments seem to form a pore in the membrane. [UniProt] |
| 预测分子量 | 76 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG59077 anti-TAP2 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59077 anti-TAP2 antibody.
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ARG59077 anti-TAP2 antibody WB image
Western blot: 25 µg of Mouse spleen lysate stained with ARG59077 anti-TAP2 antibody at 1:1000 dilution.
