ARG45596

anti-T Plastin / PLS3 antibody

anti-T Plastin / PLS3 antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes T Plastin / PLS3
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 T Plastin / PLS3
抗原物种 Human
抗原 Recombinant protein containing to human T Plastin / PLS3.
偶联标记 Un-conjugated
別名 PLS3; Plastin 3; T-Plastin; Plastin-3; Plastin 3 (T Isoform); T Fimbrin; T Plastin; T-Fimbrin; BMND18; DIH5

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IP2-4 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 71 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 102866 Mouse PLS3

GeneID: 5358 Human PLS3

Swiss-port # P13797 Human Plastin-3

Swiss-port # Q99K51 Mouse Plastin-3

基因名称 PLS3
全名 Plastin 3
背景介绍 Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
生物功能 Actin-bundling protein. [UniProt]
细胞定位 Cytoplasm; Nucleus. [UniProt]
预测分子量 71 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45596 anti-T Plastin / PLS3 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45596 anti-T Plastin / PLS3 antibody at 5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Hela and A549 stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody IP image

    Immunoprecipitation: Hela lysate immunoprecipitated with 2 μg ARG45596 anti-T Plastin / PLS3 antibody.

  • ARG45596 anti-T Plastin / PLS3 antibody FACS image

    Flow Cytometry: PC-3 stained with ARG45596 anti-T Plastin / PLS3 antibody at 1 µg/10^6 cells dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Rat stomach stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Mouse stomach stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.