ARG44748

anti-Synaptotagmin antibody

anti-Synaptotagmin antibody for Immunoprecipitation,Western blot and Human,Rat

概述

产品描述 Mouse Monoclonal antibody recognizes Synaptotagmin
反应物种 Hu, Rat
应用 IP, WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG2b
靶点名称 Synaptotagmin
抗原物种 Human
偶联标记 Un-conjugated
別名 P65; Synaptotagmin I; SytI; SVP65; SYT; p65; Synaptotagmin-1

应用说明

应用建议
应用 推荐稀释比
IP10 µg/mL
WB1 µg/mL
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Protein A purification
缓冲液 PBS with 0.09% sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25716 Rat SYT1

GeneID: 6857 Human SYT1

Swiss-port # P21579 Human Synaptotagmin-1

Swiss-port # P21707 Rat Synaptotagmin-1

基因名称 SYT1
全名 synaptotagmin I
背景介绍 The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse
生物功能 May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. [UniProt]
预测分子量 83 kDa
翻译后修饰 Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

检测图片 (2) Click the Picture to Zoom In

  • ARG44748 anti-Synaptotagmin antibody WB image

    Western blot: Rat brain stained with ARG44748 anti-Synaptotagmin antibody at 1 µg/mL dilution.

  • ARG44748 anti-Synaptotagmin antibody IP image

    Immunoprecipitation: Rat brain lysate immunoprecipitated with 2.5 µg of ARG44748 anti-Synaptotagmin antibody.