ARG52431
anti-Synapsin 1 phospho (Ser549) antibody
anti-Synapsin 1 phospho (Ser549) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Synapsin 1 phospho (Ser549) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Dog, NHuPrm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Synapsin 1 |
| 抗原物种 | Rat |
| 抗原 | KLH-conjugated phosphospecific peptide around Ser549 of Rat Synapsin 1. |
| 偶联标记 | Un-conjugated |
| 別名 | SYNI; Brain protein 4.1; Synapsin-1; SYN1a; SYN1b; Synapsin I |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Syn1 |
| 全名 | synapsin I |
| 背景介绍 | Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. |
| 生物功能 | Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. [UniProt] |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 74 kDa |
| 翻译后修饰 | Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles. |
检测图片 (1) Click the Picture to Zoom In
-
ARG52431 anti-Synapsin 1 phospho (Ser549) antibody WB image
Western blot: Rat cortex lysate showing specific immunolabeling of the ~78 kDa Synapsin 1 phosphorylated at Ser549 (Control) stained with ARG52431 anti-Synapsin 1 phospho (Ser549) antibody. Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase).
