ARG52426
anti-Synapsin 1 antibody
anti-Synapsin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Synapsin 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Synapsin 1 |
| 抗原物种 | Bovine |
| 抗原 | Native protein purified from bovine brain |
| 偶联标记 | Un-conjugated |
| 別名 | SYNI; Brain protein 4.1; Synapsin-1; SYN1a; SYN1b; Synapsin I |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~78k synapsin I doublet in Western blots of Rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to geneRate the antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SYN1 |
| 全名 | synapsin I |
| 背景介绍 | Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 74 kDa |
| 翻译后修饰 | Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles. |
检测图片 (2) Click the Picture to Zoom In
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ARG52426 anti-Synapsin 1 antibody ICC/IF image
Immunofluorescence: Cultured Mouse caudate neurons stained with ARG52426 anti-Synapsin 1 antibody (green) at 1:1000 dilution and anti-MAP antibody (red).
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ARG52426 anti-Synapsin 1 antibody WB image
Western blot: 10 ug of Rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin 1 doublet protein stained with ARG52426 anti-Synapsin 1 antibody.
