ARG63696
anti-Survivin antibody
anti-Survivin antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Survivin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cat, Cow, Dog, Pig |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Survivin |
| 抗原物种 | Human |
| 抗原 | CVRRAIEQLAAMD |
| 偶联标记 | Un-conjugated |
| 別名 | API4; Apoptosis inhibitor 4; EPR-1; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O15392 Human Baculoviral IAP repeat-containing protein 5 |
|---|---|
| 背景介绍 | This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody |
| 预测分子量 | 16 kDa |
| 翻译后修饰 | Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting. In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis. Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation. |
检测图片 (1) Click the Picture to Zoom In
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ARG63696 anti-Survivin antibody WB image
Western blot: 35 µg of Molt-4 lysate stained with ARG63696 anti-Survivin antibody at 0.01 µg/ml dilution.
