ARG54373

anti-Survivin antibody

anti-Survivin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes Survivin
反应物种 Ms
应用 IHC-P, WB
特异性 This antibody recognizes mouse Survivin (17kDa).
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Survivin
抗原物种 Mouse
抗原 Synthetic peptide corresponding to aa 128-140 of mouse Survivin (accession no. BAA28266).
偶联标记 Un-conjugated
別名 API4; Apoptosis inhibitor 4; EPR-1; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5

应用说明

应用建议
应用 推荐稀释比
IHC-P10-20 μg/mL
WB1 μg/mL
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse spleen

属性

形式 Liquid
纯化 Immunoaffinity chroma-tography
缓冲液 PBS (pH 7.4) and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11799 Mouse BIRC5

Swiss-port # O70201 Mouse Baculoviral IAP repeat-containing protein 5

基因名称 Birc5
全名 baculoviral IAP repeat-containing 5
背景介绍 Apoptosis can be prevented by the inhibitor of apoptosis proteins (IAPs). IAPs constitute a conserved gene family that binds to and inhibits cell death proteases. A novel IAP has been identified and designated Survivin. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is expressed predominantly in tissues of embryos, transformed cell lines, and many human cancers.
生物功能 Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7 (By similarity). [UniProt]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody
预测分子量 16 kDa
翻译后修饰 Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

检测图片 (2) Click the Picture to Zoom In

  • ARG54373 anti-Survivin antibody WB image

    Western Blot: mouse spleen stained with ARG54373 anti-Survivin antibody at 1 μg/ml dilution.

  • ARG54373 anti-Survivin antibody IHC-P image

    Immunohistochemistry: mouse spleen stained with ARG54373 anti-Survivin antibody at 10 μg/ml dilution.