ARG45753
anti-Sufu antibody
anti-Sufu antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Sufu |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Sufu |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human Sufu. |
| 偶联标记 | Un-conjugated |
| 別名 | suppressor of fused homolog (Drosophila); Suppressor of fused homolog; SUFUXL; PRO1280; SUFUH |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 54 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SUFU |
| 全名 | suppressor of fused homolog (Drosophila) |
| 背景介绍 | SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700) |
| 生物功能 | Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm; Nucleus. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45753 anti-Sufu antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45753 anti-Sufu antibody at 5 μg/ml dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: HEL stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
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ARG45753 anti-Sufu antibody FACS image
Flow Cytometry: U937 stained with ARG45753 anti-Sufu antibody at 1 µg/10^6 cells dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: Rat brain stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
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ARG45753 anti-Sufu antibody WB image
Western blot: Mouse brain stained with ARG45753 anti-Sufu antibody at 0.5 μg/ml dilution.
