ARG53612

anti-Stathmin antibody [SP49]

anti-Stathmin antibody [SP49] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Neuroscience antibody; Signaling Transduction antibody
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概述

产品描述 Rabbit Monoclonal antibody [SP49] recognizes Stathmin
反应物种 Hu
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SP49
同位型 IgG
靶点名称 Stathmin
抗原物种 Human
抗原 Synthetic peptide derived from internal region of human stathmin.
偶联标记 Un-conjugated
別名 PP17; Prosolin; Stathmin; Protein Pr22; PR22; Lag; C1orf215; PP19; pp19; SMN; OP18; Leukemia-associated phosphoprotein p18; LAP18; pp17; Oncoprotein 18; Phosphoprotein p19; Op18; Metablastin

应用说明

应用建议
应用 推荐稀释比
FACSAssay-Dependent
IHC-P1:100
WB1:25 - 1:100
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Tonsil, NIH/3T3

属性

形式 Liquid
纯化 Purified by protein A/G
缓冲液 PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
抗菌剂 < 0.1% Sodium azide
稳定剂 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3925 Human STMN1

Swiss-port # P16949 Human Stathmin

基因名称 STMN1
全名 stathmin 1
背景介绍 This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
生物功能 Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity). [UniProt]
细胞定位 Cytoplasm
研究领域 Neuroscience antibody; Signaling Transduction antibody
预测分子量 17 kDa
翻译后修饰 Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.

检测图片 (16) Click the Picture to Zoom In

  • ARG53612 anti-Stathmin antibody [SP49] WB image

    Western Blot: NIH3T3 stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human B-Cell Lymphoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Stomach Adenocarcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Thymus stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Tonsil stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Tonsil stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Lymph Node stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Breast Ductal Cell Carcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Colon Adenocarcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Endometrium Adenocarcinoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human HK Lymphoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Melanoma stained with Stathmin antibody [SP49] (ARG53612)

  • ARG53612 anti-Stathmin antibody [SP49] IHC-P image

    Immunohistochemistry: Human Ovarian Adenocarcinoma stained with Stathmin antibody [SP49] (ARG53612)

客户反馈

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anti-Stathmin antibody [SP49]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC