ARG58134

anti-Stathmin 1 antibody

Datasheet

订购信息

包装

价格 (USD)

50 μg

339

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Rabbit Polyclonal antibody recognizes Stathmin 1
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Stathmin 1
抗原物种	Human
抗原	Synthetic peptide of Human Stathmin 1. (ASSDIQVKELEKRASGQAFELILSPRSKESVPE)
偶联标记	Un-conjugated
別名	PP17; Prosolin; Stathmin; Protein Pr22; PR22; Lag; C1orf215; PP19; pp19; SMN; OP18; Leukemia-associated phosphoprotein p18; LAP18; pp17; Oncoprotein 18; Phosphoprotein p19; Op18; Metablastin

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 3 µg/10^6 cells
ICC/IF	2 - 4 µg/ml
IHC-P	0.5 - 2 µg/ml
WB	0.1 - 0.5 µg/ml

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min or EDTA buffer (pH 8.0) for 20 min, followed by cooling at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

Rat brain, Rat testis, Mouse brain, Mouse testis, MDA-MB-453, SH-SY5Y and Raji

实际分子量

~ 20 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS, 0.025% Sodium azide and 2.5% BSA.
抗菌剂	0.025% Sodium azide
稳定剂	2.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 16765 Mouse STMN1 GeneID: 29332 Rat STMN1 GeneID: 3925 Human STMN1 Swiss-port # P13668 Rat Stathmin Swiss-port # P16949 Human Stathmin Swiss-port # P54227 Mouse Stathmin More
基因名称	STMN1
全名	stathmin 1
背景介绍	This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
生物功能	Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity). [UniProt]
细胞定位	Cytoplasmic. [UniProt]
预测分子量	17 kDa
翻译后修饰	Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. [UniProt]

检测图片 (13) Click the Picture to Zoom In

ARG58134 anti-Stathmin 1 antibody ICC/IF image

Immunofluorescence: MCF7 cells stained with ARG58134 anti-Stathmin 1 antibody (red). DAPI (blue) for nuclear staining.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG58134 anti-Stathmin 1 antibody. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse testis tissue. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody WB image

Western blot: Mouse brain, Mouse testis, MM231 and MCF7 cell lysates stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody WB image

Western blot: Rat brain, Rat testis, Mouse brain, Mouse testis, MDA-MB-453, SH-SY5Y and Raji cell lysates stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody FACS image

Flow Cytometry: ThP-1 cells were blocked with goat sera and stained with ARG58134 anti-Stathmin 1 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse testis stained with ARG58134 anti-Stathmin 1 antibody. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placental tissue. Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat brain stained with ARG58134 anti-Stathmin 1 antibody. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse brain tissue. Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat brain tissue. Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG58134 anti-Stathmin 1 antibody.
ARG58134 anti-Stathmin 1 antibody FACS image

Flow Cytometry: Raw264.7 cells were blocked with goat sera and stained with ARG58134 anti-Stathmin 1 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
ARG58134 anti-Stathmin 1 antibody FACS image

Flow Cytometry: RH-35 cells were blocked with goat sera and stained with ARG58134 anti-Stathmin 1 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).