ARG54040
anti-Smad 5 antibody (C-term)
anti-Smad 5 antibody (C-term) for Flow cytometry,ICC/IF,Western blot and Human
Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Smad 5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | Smad 5 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | DWFC; Smad5; JV5-1; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; hSmad5; MADH5; SMAD 5; MAD homolog 5; SMAD family member 5 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q99717 Human Mothers against decapentaplegic homolog 5 |
|---|---|
| 基因名称 | SMAD5 |
| 全名 | SMAD family member 5 |
| 背景介绍 | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| 生物功能 | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus |
| 研究领域 | Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
检测图片 (3) Click the Picture to Zoom In
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ARG54040 anti-Smad 5 antibody (C-term) ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:75 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54040 anti-Smad 5 antibody (C-term) WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:1000 dilution.
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ARG54040 anti-Smad 5 antibody (C-term) FACS image
Flow Cytometry: Jurkat cells stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:100 dilution (right histogram) or isotype control (left histogram), followed by incubation with FITC labelled secondary antibody.
客户反馈
Excellent
anti-Smad 5 antibody (C-term)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:75
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
anti-Smad 5 antibody (C-term)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:75
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Good
anti-Smad 5 antibody (C-term)
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
