anti-Smad 4 antibody

anti-Smad 4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
publication_link 参考文献1


产品描述 Rabbit Polyclonal antibody recognizes Smad 4
反应物种 Hu, Ms, Rat
预测物种 Rat
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Smad 4
抗原物种 Human
抗原 Recombinant protein of human SMAD4 (NP_005350.1).
偶联标记 Un-conjugated
別名 Smad4; Mothers against decapentaplegic homolog 4; SMAD family member 4; MADH4; hSMAD4; DPC4; JIP; MAD homolog 4; SMAD 4; MYHRS; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4


应用 推荐稀释比
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 17128 Mouse SMAD4

GeneID: 4089 Human SMAD4

GeneID: 50554 Rat SMAD4

基因名称 SMAD4
全名 SMAD family member 4
背景介绍 Smad4 gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]
生物功能 In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]
产品亮点 Related products:
Smad 4 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Therapeutic strategies against PDAC
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 60 kDa
翻译后修饰 Phosphorylated by PDPK1.
Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.

检测图片 (3) Click the Picture to Zoom In

  • ARG54741 anti-Smad 4 antibody WB image

    Western blot: 30 µg of HCT116 cell lysate stained with ARG54741 anti-Smad 4 antibody at 1:500 dilution.

  • ARG54741 anti-Smad 4 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54741 anti-Smad 4 antibody.

  • ARG54741 anti-Smad 4 antibody IHC-P image

    Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded Mouse testis tissue stained with ARG54741 anti-Smad 4 antibody.


nuts_pic      Good

anti-Smad 4 antibody


Sample:HCT116 cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC


Mechanism of epithelial‑mesenchymal transition inhibited by miR‑203 in non‑small cell lung cancer.

WB / Human

Huang Weicong et al.
Oncol Rep.,  (2020)