ARG51848
anti-Smad 1 phospho (Ser206) antibody
anti-Smad 1 phospho (Ser206) antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Smad 1 phospho (Ser206) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Smad 1 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 206 (P-H-S(p)-P-T) derived from Human Smad1. |
| 偶联标记 | Un-conjugated |
| 別名 | MADR1; Mad-related protein 1; Transforming growth factor-beta-signaling protein 1; Mothers against decapentaplegic homolog 1; MADH1; SMAD 1; Smad1; JV4-1; JV41; BSP-1; hSMAD1; BSP1; Mothers against DPP homolog 1; MAD homolog 1; SMAD family member 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P70340 Mouse Mothers against decapentaplegic homolog 1 Swiss-port # Q15797 Human Mothers against decapentaplegic homolog 1 |
|---|---|
| 基因名称 | SMAD1 |
| 全名 | SMAD family member 1 |
| 背景介绍 | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). |
| 生物功能 | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30155 Phospho Smad1 for Activation / Inhibition site Antibody Panel Related products: Smad 1 antibodies; Smad 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG51848 anti-Smad 1 phospho (Ser206) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with PMA stained with ARG51848 anti-Smad 1 phospho (Ser206) antibody.
