ARG57123
anti-Smac / Diablo antibody [19F2]
anti-Smac / Diablo antibody [19F2] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [19F2] recognizes Smac / Diablo |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 19F2 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Smac / Diablo |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 56-239 of Human Smac / Diablo |
| 偶联标记 | Un-conjugated |
| 別名 | Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DIABLO |
| 全名 | diablo, IAP-binding mitochondrial protein |
| 背景介绍 | This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] |
| 生物功能 | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt] |
| 预测分子量 | 27 kDa |
| 翻译后修饰 | Ubiquitinated by BIRC7/livin. |
检测图片 (4) Click the Picture to Zoom In
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ARG57123 anti-Smac / Diablo antibody [19F2] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57123 anti-Smac / Diablo antibody [19F2] FACS image
Flow Cytometry: HeLa cell line stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57123 anti-Smac / Diablo antibody [19F2] WB image
Western blot: 40 µg of Jurkat cell lysate stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:3000.
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ARG57123 anti-Smac / Diablo antibody [19F2] WB image
Western blot: 40 µg of 1) 293T, 2) A431, 3) Hep3B, and 4) MCF7 cell lysates stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:1000.
