anti-Sialyl Tn antibody [STn 219] (FITC)

anti-Sialyl Tn antibody [STn 219] (FITC) for Flow cytometry,ICC/IF,Immunohistochemistry and Human

Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody


产品描述 FITC-conjugated Mouse Monoclonal antibody [STn 219] recognizes Sialyl Tn
反应物种 Hu
特异性 This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
宿主 Mouse
克隆 Monoclonal
克隆号 STn 219
同位型 IgG1, kappa
靶点名称 Sialyl Tn
抗原 Ovine submaxillary mucin (OSM)
抗原表位 NeuAc α GalNacOSer/Thr
偶联标记 FITC


应用 推荐稀释比
FACS1:200 - 1:1000
ICC/IF1:10 - 1:20
IHC1:50 - 1:100
应用说明 IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears.
IHC: Use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature (1:50 - 1:100).
IF, FITC~conjugated antibodies 10-20 µg/ml (1:10 - 1:20), incubatein the dark for 2 hours at RT or overnight at 4°C.
Flow: FITC~conjugated antibodies, 0.2-1.0 µg/0.1 ml (1:200 - 1:1000).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human gastrointestinal tumor, prostate, ovary ca


形式 Liquid
缓冲液 PBS (pH 7.4), 1% BSA and 0.05% Sodium azide
抗菌剂 0.05% Sodium azide
稳定剂 1% BSA
浓度 0.2 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.


背景介绍 Sialsyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialsyl Tn antigen has a limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas.
细胞定位 Cytoplasmic, apical membrane, luminal
产品亮点 Related Antibody Duos and Panels:
ARG30015 Tn Antigen Antibody Duo (sTn, Tn)
Related products:
Sialyl Tn antibodies; Sialyl Tn Duos / Panels;
研究领域 Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody
预测分子量 several HM weight glycoproteins