anti-Sialyl Tn antibody [STn 219]
anti-Sialyl Tn antibody [STn 219] for Flow cytometry,ICC/IF,Immunohistochemistry and Human
Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody
|产品描述||Mouse Monoclonal antibody [STn 219] recognizes Sialyl Tn|
|应用||FACS, ICC/IF, IHC|
|特异性||This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.|
|抗原||Ovine submaxillary mucin (OSM)|
|抗原表位||NeuAc α GalNacOSer/Thr|
|应用说明||IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears.
IHC: Use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature (1:50 - 1:100).
IF, FITC~conjugated antibodies 10-20 µg/ml (1:10 - 1:20), incubatein the dark for 2 hours at RT or overnight at 4°C.
Flow: FITC~conjugated antibodies, 0.2-1.0 µg/0.1 ml (1:200 - 1:1000).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|阳性对照||Human gastrointestinal tumor, prostate, ovary ca|
|缓冲液||PBS (pH 7.4), 1% BSA and 0.05% Sodium azide|
|抗菌剂||0.05% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|背景介绍||Sialsyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialsyl Tn antigen has a limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas.|
|细胞定位||Cytoplasmic, apical membrane, luminal|
|产品亮点||Related Antibody Duos and Panels:
ARG30015 Tn Antigen Antibody Duo (sTn, Tn)
Sialyl Tn antibodies; Sialyl Tn Duos / Panels; Anti-Mouse IgG secondary antibodies;
|研究领域||Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody|
|预测分子量||several HM weight glycoproteins|
检测图片 (1) Click the Picture to Zoom In