ARG45780

anti-Sec31A antibody

anti-Sec31A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes Sec31A
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Sec31A
抗原物种 Human
抗原 Recombinant protein containing to human Sec31A.
偶联标记 Un-conjugated
別名 SEC31A; SEC31 Homolog A, COPII Coat Complex Component; ABP125; ABP130; KIAA0905; SEC31L1; Protein Transport Protein Sec31A; SEC31-Related Protein A; SEC31-Like Protein 1; Web1-Like Protein; SEC31 Homolog A, COPII Coating Complex Component; Sec31 Homolog A (S. Cerevisiae); SEC31-Like 1 (S. Cerevisiae); Yeast Sec31p Homolog; HSPC275; HSPC334; NEDSOSB; HPBKS

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 133 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 22872 Human SEC31A

GeneID: 69162 Mouse SEC31A

GeneID: 93646 Rat SEC31A

基因名称 SEC31A
全名 SEC31 Homolog A, COPII Coat Complex Component
背景介绍 The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
生物功能 Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). [UniProt]
细胞定位 Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Membrane. [UniProt]
预测分子量 133 kDa
翻译后修饰 Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (8) Click the Picture to Zoom In

  • ARG45780 anti-Sec31A antibody IHC-P image

    Immunohistochemistry: Human renal cancer stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45780 anti-Sec31A antibody at 5 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody WB image

    Western blot: HepG2 and U-87MG stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45780 anti-Sec31A antibody at 1 µg/10^6 cells dilution.

  • ARG45780 anti-Sec31A antibody IHC-P image

    Immunohistochemistry: Rat colon stained with ARG45780 anti-Sec31A antibody at 2 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody WB image

    Western blot: PC-12 stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.

  • ARG45780 anti-Sec31A antibody WB image

    Western blot: NIH/3T3 stained with ARG45780 anti-Sec31A antibody at 0.5 μg/ml dilution.