ARG45217

anti-SUN2 antibody

anti-SUN2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes SUN2
反应物种 Hu
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SUN2
抗原物种 Human
抗原 Recombinant protein containing to human SUN2.
偶联标记 Un-conjugated
別名 SUN2; Sad1 And UNC84 Domain Containing 2; UNC84B; SUN Domain-Containing Protein 2; Sad1/Unc-84 Protein-Like 2; Protein Unc-84 Homolog B; Rab5-Interacting Protein; Rab5IP; Unc-84 Homolog B (C. Elegans); Sad1 Unc-84 Domain Protein 2; Nuclear Envelope Protein; Unc-84 Homolog B; KIAA0668; RAB5IP; FRIGG

应用说明

应用建议
应用 推荐稀释比
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 80 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25777 Human SUN2

Swiss-port # Q9UH99 Human SUN domain-containing protein 2

基因名称 SUN2
全名 Sad1 And UNC84 Domain Containing 2
背景介绍 SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010]
生物功能 As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.. [UniProt]
细胞定位 Endosome; Membrane; Nucleus [UniProt]
预测分子量 80 kDa
翻译后修饰 Disulfide bond; Glycoprotein; Phosphoprotein. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45217 anti-SUN2 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG45217 anti-SUN2 antibody at 2 μg/ml dilution.

  • ARG45217 anti-SUN2 antibody ICC/IF image

    Immunofluorescence: A431 stained with ARG45217 anti-SUN2 antibody at 5 ug/ml dilution.

  • ARG45217 anti-SUN2 antibody WB image

    Western blot: HepG2 and Hela stained with ARG45217 anti-SUN2 antibody at 0.5 μg/ml dilution.