ARG66700
anti-STAT6 phospho (Tyr641) antibody
anti-STAT6 phospho (Tyr641) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes STAT6 phospho (Tyr641) |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STAT6 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Tyr641 of Human STAT6. |
| 偶联标记 | Un-conjugated |
| 別名 | D12S1644; STAT6B; STAT6C; Signal transducer and activator of transcription 6; IL-4-STAT; IL-4 Stat |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 95 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P42226 Human Signal transducer and activator of transcription 6 Swiss-port # P52633 Mouse Signal transducer and transcription activator 6 |
|---|---|
| 基因名称 | STAT6 |
| 全名 | signal transducer and activator of transcription 6, interleukin-4 induced |
| 背景介绍 | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| 生物功能 | Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. [UniProt] |
| 预测分子量 | 94 kDa |
| 翻译后修饰 | Tyrosine phosphorylated following stimulation by IL4/interleukin-4 and IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66700 anti-STAT6 phospho (Tyr641) antibody WB image
Western blot: HeLa and Jurkat cells were untreated or treated with IFN 2500U/ml for 30 minutes, overnight at 4°C. Cell lysates were stained with ARG66700 anti-STAT6 phospho (Tyr641) antibody.
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ARG66700 anti-STAT6 phospho (Tyr641) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG66700 anti-STAT6 phospho (Tyr641) antibody. The picture on the right is blocked with the phospho peptide.
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ARG66700 anti-STAT6 phospho (Tyr641) antibody WB image
Western blot: HeLa cells treated with IL4. Cell lysates were stained with ARG66700 anti-STAT6 phospho (Tyr641) antibody. The lane on the right is blocked with the phospho peptide.
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ARG66700 anti-STAT6 phospho (Tyr641) antibody WB image
Western blot: HeLa cell lysate stained with ARG66700 anti-STAT6 phospho (Tyr641) antibody.
