ARG63646
anti-STAP2 / BKS antibody
anti-STAP2 / BKS antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes STAP2 / BKS |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STAP2 / BKS |
| 抗原物种 | Human |
| 抗原 | C-ELQKKLEKRRALEH |
| 偶联标记 | Un-conjugated |
| 別名 | Signal-transducing adaptor protein 2; BRK substrate; STAP-2; Breast tumor kinase substrate; BKS |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UGK3 Human Signal-transducing adaptor protein 2 |
|---|---|
| 背景介绍 | This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| 研究领域 | Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC. |
检测图片 (4) Click the Picture to Zoom In
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ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63646 anti-STAP2 / BKS antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63646 anti-STAP2 / BKS antibody WB image
Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63646 anti-STAP2 / BKS antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.
