ARG58234
anti-STAMBP / AMSH antibody
anti-STAMBP / AMSH antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes STAMBP / AMSH |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STAMBP / AMSH |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 100-270 of Human STAMBP (NP_998787.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Endosome-associated ubiquitin isopeptidase; AMSH; Associated molecule with the SH3 domain of STAM; MICCAP; STAM-binding protein; EC 3.4.19.- |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | MCF7 | ||||||||||
| 实际分子量 | 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | STAMBP |
| 全名 | STAM binding protein |
| 背景介绍 | Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (By similarity). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways. [UniProt] |
| 细胞定位 | Nucleus, Membrane, Peripheral membrane protein, Cytoplasm, Early endosome. [UniProt] |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Phosphorylated after BMP type I receptor activation. Ubiquitinated by SMURF2 in the presence of RNF11. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58234 anti-STAMBP / AMSH antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58234 anti-STAMBP / AMSH antibody.
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ARG58234 anti-STAMBP / AMSH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG58234 anti-STAMBP / AMSH antibody at 1:100 dilution.
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ARG58234 anti-STAMBP / AMSH antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG58234 anti-STAMBP / AMSH antibody at 1:1000 dilution.
