ARG58270
anti-SSRP1 antibody
anti-SSRP1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SSRP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SSRP1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-300 of Human SSRP1 (NP_003137.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Structure-specific recognition protein 1; Chromatin-specific transcription elongation factor 80 kDa subunit; hSSRP1; FACT complex subunit SSRP1; T160; FACT 80 kDa subunit; FACTp80; FACT80; Facilitates chromatin transcription complex subunit SSRP1; Facilitates chromatin transcription complex 80 kDa subunit; FACT; Recombination signal sequence recognition protein 1 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | SW620 | ||||||||
| 实际分子量 | 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SSRP1 |
| 全名 | structure specific recognition protein 1 |
| 背景介绍 | The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. [UniProt] |
| 细胞定位 | Chromosome, Nucleus, nucleolus. [UniProt] |
| 预测分子量 | 81 kDa |
| 翻译后修饰 | Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity. Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein. Sumoylated. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG58270 anti-SSRP1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58270 anti-SSRP1 antibody.
-
ARG58270 anti-SSRP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophageal cancer stained with ARG58270 anti-SSRP1 antibody at 1:100 dilution.
-
ARG58270 anti-SSRP1 antibody WB image
Western blot: 25 µg of SW620 cell lysate stained with ARG58270 anti-SSRP1 antibody at 1:1000 dilution.
