ARG42951
anti-SSR3 antibody
anti-SSR3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SSR3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SSR3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 8-23 of Human SSR3. (KQQSEEDLLLQDFSRN) |
| 偶联标记 | Un-conjugated |
| 別名 | TRAPG; Translocon-associated protein subunit gamma; Signal sequence receptor subunit gamma; TRAP-gamma; SSR-gamma |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 21 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SSR3 |
| 全名 | signal sequence receptor, gamma (translocon-associated protein gamma) |
| 背景介绍 | The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR is comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. The first two are glycosylated subunits and the latter two are non-glycosylated subunits. This gene encodes the gamma subunit, which is predicted to span the membrane four times. [provided by RefSeq, Aug 2010] |
| 生物功能 | TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 21 kDa |
检测图片 (9) Click the Picture to Zoom In
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ARG42951 anti-SSR3 antibody ICC image
Immunocytochemistry: HeLa cells were blocked with 10% goat serum and then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostatic cancer tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody WB image
Western blot: 50 µg of Rat liver, 50 µg of Rat spleen, 40 µg of A431, 40 µg of HeLa, 40 µg of U87 and 40 µg of SMMC-7721 whole cell lysates stained with ARG42951 anti-SSR3 antibody at 0.5 µg/ml dilution.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Mouse brain tissue stained with ARG42951 anti-SSR3 antibody at 2 μg/mL dilution.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue stained with ARG42951 anti-SSR3 antibody.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42951 anti-SSR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42951 anti-SSR3 antibody at 1 µg/ml dilution, overnight at 4°C.
