ARG56539
anti-SREBP2 antibody
anti-SREBP2 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SREBP2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SREBP2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around an internal region of Human SREBP-2. |
| 偶联标记 | Un-conjugated |
| 別名 | Class D basic helix-loop-helix protein 2; Sterol regulatory element-binding protein 2; bHLHd2; Sterol regulatory element-binding transcription factor 2; SREBP2; SREBP-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | TBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.1% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SREBF2 |
| 全名 | sterol regulatory element binding transcription factor 2 |
| 背景介绍 | This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. [UniProt] |
| 预测分子量 | 124 kDa |
| 翻译后修饰 | At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. |
检测图片 (1) Click the Picture to Zoom In
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ARG56539 anti-SREBP2 antibody WB image
Western blot: 1) 60 µg of Human fibroblast cell lysate, 2) 60 µg of Rat brown fat homogenate, and 3) 60 µg of Rat testis supernatant stained with ARG56539 anti-SREBP2 antibody.
文献引用
Study on the mechanism of the regulatory effects of Guanxinkang and its composite formulas on hyperlipidemia based on PERK-eIF2α signaling pathway.
WB / Mouse
