ARG45285

anti-SPRED2 antibody

anti-SPRED2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Polyclonal antibody recognizes SPRED2
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SPRED2
抗原物种 Human
抗原 Recombinant protein containing to human SPRED2.
偶联标记 Un-conjugated
別名 SPRED2; Sprouty Related EVH1 Domain Containing 2; Spred-2; Sprouty-Related, EVH1 Domain-Containing Protein 2; FLJ21897; FLJ31917; Sprouty Protein With EVH-1 Domain 2, Related Sequence; NS14

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 53 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 114716 Mouse SPRED2

GeneID: 200734 Human SPRED2

GeneID: 305539 Rat SPRED2

基因名称 SPRED2
全名 Sprouty Related EVH1 Domain Containing 2
背景介绍 SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]
生物功能 Negatively regulates Ras signaling pathways and downstream activation of MAP kinases. [UniProt]
细胞定位 Cell membrane; Cytoplasm; Cytoplasmic vesicle; Membrane. [UniProt]
预测分子量 48 kDa
翻译后修饰 Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45285 anti-SPRED2 antibody ICC/IF image

    Immunofluorescence: U20S stained with ARG45285 anti-SPRED2 antibody at 5 ug/ml dilution.

  • ARG45285 anti-SPRED2 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45285 anti-SPRED2 antibody at 1 µg/10^6 cells dilution.

  • ARG45285 anti-SPRED2 antibody WB image

    Western blot: Rat brain stained with ARG45285 anti-SPRED2 antibody at 0.5 μg/ml dilution.

  • ARG45285 anti-SPRED2 antibody WB image

    Western blot: Mouse brain stained with ARG45285 anti-SPRED2 antibody at 0.5 μg/ml dilution.