ARG56527

anti-SPHK1 antibody

anti-SPHK1 antibody for ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes SPHK1
反应物种 Hu
预测物种 Ms, Pig, Rb, frg
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SPHK1
抗原物种 Human
抗原 Synthetic peptide around an internal region of Human SPHK1.
偶联标记 Un-conjugated
別名 SK 1; SPK 1; EC 2.7.1.91; SPHK; Sphingosine kinase 1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:80
WB1:200
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 8877 Human SPHK1

Swiss-port # Q9NYA1 Human Sphingosine kinase 1

基因名称 SPHK1
全名 sphingosine kinase 1
背景介绍 The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
生物功能 Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. [UniProt]
预测分子量 43 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG56527 anti-SPHK1 antibody WB image

    Western blot: 1) Precision Plus Protein Standard, 2) 10 µg of SPHK 1 transfected cell lysates, 3) 20 µg of SPHK 1 transfected cell lysates, 4) 25 µg of Mouse heart homogenate, and 5) 50 µg of Mouse heart homogenate stained with ARG56527 anti-SPHK1 antibody.