ARG45278

anti-SPDEF / PSE antibody

anti-SPDEF / PSE antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Polyclonal antibody recognizes SPDEF / PSE
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SPDEF / PSE
抗原物种 Human
抗原 Recombinant protein containing to human SPDEF / PSE.
偶联标记 Un-conjugated
別名 SPDEF; SAM Pointed Domain Containing ETS Transcription Factor; PDEF; BA375E1.3; SAM Pointed Domain-Containing Ets Transcription Factor; Prostate Epithelium-Specific Ets Transcription Factor; Prostate-Derived Ets Factor; Prostate-Specific Ets; PSE

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 44 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25803 Human SPDEF

Swiss-port # O95238 Human SAM pointed domain-containing Ets transcription factor

基因名称 SPDEF / PSE
全名 SAM Pointed Domain Containing ETS Transcription Factor
背景介绍 The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
生物功能 May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 38 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG45278 anti-SPDEF / PSE antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45278 anti-SPDEF / PSE antibody at 5 ug/ml dilution.

  • ARG45278 anti-SPDEF / PSE antibody WB image

    Western blot: MCF-7 and PC-3 stained with ARG45278 anti-SPDEF / PSE antibody at 0.5 μg/ml dilution.

  • ARG45278 anti-SPDEF / PSE antibody FACS image

    Flow Cytometry: MCF-7 stained with ARG45278 anti-SPDEF / PSE antibody at 1 µg/10^6 cells dilution.