ARG65128

anti-SPARTIN antibody

anti-SPARTIN antibody for ICC/IF,Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes SPARTIN
反应物种 Hu
应用 ICC/IF, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 SPARTIN
抗原物种 Human
抗原 C-EASGTDVKQLDQGNK
偶联标记 Un-conjugated
別名 Spastic paraplegia 20 protein; SPARTIN; Trans-activated by hepatitis C virus core protein 1; Spartin; TAHCCP1

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay - dependent
WB1 - 3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 23111 Human SPG20

Swiss-port # Q8N0X7 Human Spartin

背景介绍 This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008]
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量 73 kDa
翻译后修饰 Ubiquitinated; ubiquitination does not require ITCH and WWP1.

检测图片 (2) Click the Picture to Zoom In

  • ARG65128 anti-SPARTIN antibody WB image

    Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG65128 anti-SPARTIN antibody at 2 µg/ml dilution.

  • ARG65128 anti-SPARTIN antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa stained with ARG65128 anti-SPARTIN antibody (2µg/ml) before (left) and after (right) introduction of siRNA.