ARG66767

anti-SOX2 antibody [SQab20200]

anti-SOX2 antibody [SQab20200] for IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab20200] recognizes SOX2
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab20200
同位型 IgG
靶点名称 SOX2
抗原物种 Human
抗原 Synthetic peptide within aa. 1-100 of Human SOX2.
偶联标记 Un-conjugated
別名 Transcription factor SOX-2; MCOPS3; ANOP3

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Lung squamous cell carcinomas tissue.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6657 Human SOX2

Swiss-port # P48431 Human Transcription factor SOX-2

基因名称 SOX2
全名 SRY (sex determining region Y)-box 2
背景介绍 This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
生物功能 Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 34 kDa
翻译后修饰 Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66767 anti-SOX2 antibody [SQab20200] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human lung squamous cell carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66767 anti-SOX2 antibody [SQab20200] at 18°C - 25°C for 30 minutes.