ARG66245
anti-SOX10 antibody [SQab1734]
anti-SOX10 antibody [SQab1734] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1734] recognizes SOX10 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1734 |
| 同位型 | IgG |
| 靶点名称 | SOX10 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 400-500 of Human SOX10. |
| 偶联标记 | Un-conjugated |
| 別名 | WS4C; PCWH; DOM; Transcription factor SOX-10; WS2E; WS4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SOX10 |
| 全名 | SRY (sex determining region Y)-box 10 |
| 背景介绍 | SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008] |
| 生物功能 | SOX10 is a transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). [UniProt] |
| 产品亮点 | Related products: SOX10 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 50 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG66245 anti-SOX10 antibody [SQab1734] ICC/IF image
Immunofluorescence: A375 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:10000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66245 anti-SOX10 antibody [SQab1734] FACS image
Flow Cytometry: A375 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. Cells were then stained with ARG66245 anti-SOX10 antibody [SQab1734] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.
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ARG66245 anti-SOX10 antibody [SQab1734] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma tissue stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:100. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
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ARG66245 anti-SOX10 antibody [SQab1734] WB image
Western blot: 10 μg of A375 cell lysate stained with ARG66245 anti-SOX10 antibody [SQab1734] at 1:1000 dilution.
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ARG66245 anti-SOX10 antibody [SQab1734] IP image
Immunoprecipitation: 0.4 mg of A375 cell lysate immunoprecipitated and stained with ARG66245 anti-SOX10 antibody [SQab1734]. 1) IP in A375 whole cell lysate, 2) Rabbit IgG instead of primary antibody in A375 whole cell lysate, and 3) A375 whole cell lysate, 10 μg (input).
