ARG43064
anti-SORBS3 / Vinexin antibody
anti-SORBS3 / Vinexin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SORBS3 / Vinexin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SORBS3 / Vinexin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human SORBS3 / Vinexin. (ASTKIPASQHTQNWSATWTKDSKRRDKRWVKYE) |
| 偶联标记 | Un-conjugated |
| 別名 | SH3-containing adapter molecule 1; Sorbin and SH3 domain-containing protein 3; SCAM-1; Vinexin; SCAM1; SH3D4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SORBS3 |
| 全名 | sorbin and SH3 domain containing 3 |
| 背景介绍 | This gene encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization, cell adhesion and migration, signaling, and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| 生物功能 | Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation by using its third SH3 domain. [UniProt] |
| 细胞定位 | Isoform Alpha: Cell junction. Cytoplasm, cytoskeleton. Note=Localized at cell-extracellular matrix junctions (By similarity). Both isoforms were localized at focal adhesion and cell-cell adhesion sites. Isoform Beta: Cell junction. Nucleus. Cytoplasm, cytoskeleton. Note=Localized at cell-extracellular matrix junctions (By similarity). Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus. [UniProt] |
| 预测分子量 | 75 kDa |
| 翻译后修饰 | Phosphorylated at Ser-530 by MAPK1/ERK2 during cell spreading. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG43064 anti-SORBS3 / Vinexin antibody ICC/IF image
Immunofluorescence: A549 cells were blocked with 10% goat serum and then stained with ARG43064 anti-SORBS3 / Vinexin antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43064 anti-SORBS3 / Vinexin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43064 anti-SORBS3 / Vinexin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43064 anti-SORBS3 / Vinexin antibody WB image
Western blot: 50 µg of sample under reducing conditions. THP-1, Rat liver, Mouse brain and HEPA1-6 whole cell lysates stained with ARG43064 anti-SORBS3 / Vinexin antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43064 anti-SORBS3 / Vinexin antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG43064 anti-SORBS3 / Vinexin antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43064 anti-SORBS3 / Vinexin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43064 anti-SORBS3 / Vinexin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43064 anti-SORBS3 / Vinexin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43064 anti-SORBS3 / Vinexin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43064 anti-SORBS3 / Vinexin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestinal lymph node tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43064 anti-SORBS3 / Vinexin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43064 anti-SORBS3 / Vinexin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43064 anti-SORBS3 / Vinexin antibody at 1 µg/ml dilution, overnight at 4°C.
