ARG54937

anti-SOD2 antibody

anti-SOD2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
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publication_link 参考文献5

概述

产品描述 Rabbit Polyclonal antibody recognizes SOD2
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SOD2
抗原物种 Human
抗原 Recombinant protein of Human SOD2 (NP_000627.2)
偶联标记 Un-conjugated
別名 MNSOD; Superoxide dismutase [Mn], mitochondrial; IPOB; EC 1.15.1.1; MVCD6

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20656 Mouse SOD2

GeneID: 24787 Rat SOD2

GeneID: 6648 Human SOD2

基因名称 SOD2
全名 superoxide dismutase 2, mitochondrial
背景介绍 This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
生物功能 Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30274 SOD1 and SOD2 Antibody Duo
Related products:
SOD2 antibodies; SOD2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量 25 kDa
翻译后修饰 Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).

检测图片 (4) Click the Picture to Zoom In

  • ARG54937 anti-SOD2 antibody WB image

    Western blot: Mouse hippocampal stained with ARG54937 anti-SOD2 antibody.

    From Ji-Ren An et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1136070, Fig. 7F.

  • ARG54937 anti-SOD2 antibody WB image

    Western blot: 30 µg of HeLa, HepG2, MDAMB231, HEK293T, Mouse heart, Mouse liver, and Rat heart lysates stained with ARG54937 anti-SOD2 antibody at 1:500 dilution.

  • ARG54937 anti-SOD2 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54937 anti-SOD2 antibody (orange) at 1:10 dilution.

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)

  • ARG54937 anti-SOD2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human oophoroma stained with ARG54937 anti-SOD2 antibody at 1:100 dilution (400x lens).

客户反馈

nuts_pic      Excellent

anti-SOD2 antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:10

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:TRITC

nuts_pic      Excellent

anti-SOD2 antibody

Application:WB

Sample:PC3 cell line

Sample Loading Amount:20µg

Primary Antibody Dilution Factor:1:1,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress

WB / Mouse

Ji-Ren An et al.
Front Cell Neurosci.,  (2023)

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Fe3O4 Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in D-Galactose-Induced Aged Mice

WB / Mouse

Zihao Xia et al.
International Journal o f Molecular Sciences,  (2022)

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Dietary Fe 3 O 4 Nanozymes Prevent the Injury of Neurons and Blood-Brain Barrier Integrity from Cerebral Ischemic Stroke.

WB / Mouse

Bing Chun Yan et al.
ACS Biomater Sci Eng.,  (2021)

publication_link

 

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The antiepileptic drug levetiracetam promotes neuroblast differentiation and expression of superoxide dismutase in the mouse hippocampal dentate gyrus via PI3K/Akt signalling.

WB / Mouse, Rat

Yan Bing-Chun et al.
Neurosci Lett.,  (2018)

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Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the D-Galactose-Induced Aging Mice via Its Antioxidant Effects.

WB, IHC-Fr / Mouse

Shen H et al.
Cell Mol Neurobiol.,  (2016)

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