anti-SOD2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Rabbit Polyclonal antibody recognizes SOD2
|反应物种||Hu, Ms, Rat|
|应用||ICC/IF, IHC-Fr, IHC-P, WB|
|抗原||Recombinant protein of Human SOD2 (NP_000627.2)|
|別名||MNSOD; Superoxide dismutase [Mn], mitochondrial; IPOB; EC 184.108.40.206; MVCD6|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|纯化||Affinity purification with immunogen.|
|缓冲液||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||superoxide dismutase 2, mitochondrial|
|背景介绍||This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]|
|生物功能||Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt]|
|产品亮点||Related Antibody Duos and Panels:
ARG30274 SOD1 and SOD2 Antibody Duo
SOD2 antibodies; SOD2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
|研究领域||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody|
|翻译后修饰||Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity).
检测图片 (4) Click the Picture to Zoom In
ARG54937 anti-SOD2 antibody WB image
Western blot: Mouse hippocampal stained with ARG54937 anti-SOD2 antibody.
From Ji-Ren An et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1136070, Fig. 7F.
ARG54937 anti-SOD2 antibody WB image
Western blot: 30 µg of HeLa, HepG2, MDAMB231, HEK293T, Mouse heart, Mouse liver, and Rat heart lysates stained with ARG54937 anti-SOD2 antibody at 1:500 dilution.
ARG54937 anti-SOD2 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54937 anti-SOD2 antibody (orange) at 1:10 dilution.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
ARG54937 anti-SOD2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oophoroma stained with ARG54937 anti-SOD2 antibody at 1:100 dilution (400x lens).
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:10
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC