ARG57160
anti-SOD1 antibody [6F1]
anti-SOD1 antibody [6F1] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [6F1] recognizes SOD1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6F1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | SOD1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-154 of Human SOD1 |
| 偶联标记 | Un-conjugated |
| 別名 | homodimer; EC 1.15.1.1; SOD; HEL-S-44; Superoxide dismutase [Cu-Zn]; ALS1; Superoxide dismutase 1; IPOA; ALS; hSod1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SOD1 |
| 全名 | superoxide dismutase 1, soluble |
| 背景介绍 | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt] |
| 预测分子量 | 16 kDa |
| 翻译后修饰 | Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity. |
检测图片 (3) Click the Picture to Zoom In
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ARG57160 anti-SOD1 antibody [6F1] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57160 anti-SOD1 antibody [6F1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57160 anti-SOD1 antibody [6F1] WB image
Western blot: 40 µg of 1) A549, 2) A431, 3) 293T, 4) PC3, and 5) U87MG cell lysates stained with ARG57160 anti-SOD1 antibody [6F1] at 1:1000.
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ARG57160 anti-SOD1 antibody [6F1] WB image
Western blot: 20 ng of 1) SOD1, and 2) SOD2 recombinant proteins stained with ARG57160 anti-SOD1 antibody [6F1] at 1:1000.
