ARG63099
anti-SOCS3 antibody [SO1]
anti-SOCS3 antibody [SO1] for Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [SO1] recognizes SOCS3 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | The clone SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SO1 |
| 同位型 | IgG2b |
| 靶点名称 | SOCS3 |
| 抗原 | Full-length SOCS3 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | SOCS-3; Cish3; CIS-3; Suppressor of cytokine signaling 3; SSI-3; SSI3; CIS3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3; ATOD4 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O14543 Human Suppressor of cytokine signaling 3 |
|---|---|
| 基因名称 | SOCS3 |
| 全名 | suppressor of cytokine signaling 3 |
| 背景介绍 | SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation. |
| 生物功能 | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity). [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. |
检测图片 (1) Click the Picture to Zoom In
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ARG63099 anti-SOCS3 antibody [SO1] WB image
Western blot: HeLa cell lysate stained with 1) Isotype mouse IgG2b control antibody or 2) ARG63099 anti-SOCS3 antibody [SO1]. .
克隆号文献
Suppressor of cytokine signalling-3 is up-regulated by androgen in prostate cancer cell lines and inhibits androgen-mediated proliferation and secretion.
