ARG55763
anti-SNRPD3 antibody
anti-SNRPD3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SNRPD3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Xenopus |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SNRPD3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 99-126 (C-terminus) of Human SNRPD3. |
| 偶联标记 | Un-conjugated |
| 別名 | Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; SMD3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | MCF7 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P62318 Human Small nuclear ribonucleoprotein Sm D3 |
|---|---|
| 基因名称 | SNRPD3 |
| 全名 | small nuclear ribonucleoprotein D3 polypeptide 18kDa |
| 背景介绍 | This gene encodes a core component of the spliceosome, which is a nuclear ribonucleoprotein complex that functions in pre-mRNA splicing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. As part of the U7 snRNP it is involved in histone 3'-end processing. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Nucleus. Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes |
| 预测分子量 | 14 kDa |
| 翻译后修饰 | Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs. |
检测图片 (3) Click the Picture to Zoom In
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ARG55763 anti-SNRPD3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55763 anti-SNRPD3 antibody.
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ARG55763 anti-SNRPD3 antibody WB image
Western blot: 35 µg of MCF7 cell lysate stained with ARG55763 anti-SNRPD3 antibody.
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ARG55763 anti-SNRPD3 antibody FACS image
Flow Cytometry: MCF7 cells stained with ARG55763 anti-SNRPD3 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
