ARG40138
anti-SNRPD1 / Sm D1 antibody
anti-SNRPD1 / Sm D1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SNRPD1 / Sm D1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SNRPD1 / Sm D1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 69-98 of Human SNRPD1. |
| 偶联标记 | Un-conjugated |
| 別名 | Sm-D autoantigen; snRNP core protein D1; Sm-D1; Small nuclear ribonucleoprotein Sm D1; HsT2456; SMD1; SNRPD |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P62314 Human Small nuclear ribonucleoprotein Sm D1 |
|---|---|
| 基因名称 | SNRPD1 |
| 全名 | small nuclear ribonucleoprotein D1 polypeptide 16kDa |
| 背景介绍 | This gene encodes a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. The protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| 生物功能 | Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Nucleus. Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes. [UniProt] |
| 预测分子量 | 13 kDa |
| 翻译后修饰 | Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40138 anti-SNRPD1 / Sm D1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG40138 anti-SNRPD1 / Sm D1 antibody.
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ARG40138 anti-SNRPD1 / Sm D1 antibody WB image
Western blot: 20 µg of K562 whole cell lysate stained with ARG40138 anti-SNRPD1 / Sm D1 antibody at 1:1000 dilution.
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ARG40138 anti-SNRPD1 / Sm D1 antibody FACS image
Flow Cytometry: HL-60 cells stained with ARG40138 anti-SNRPD1 / Sm D1 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
