ARG41354
anti-SNAP23 antibody
anti-SNAP23 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SNAP23 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Dog |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SNAP23 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 192-211 of Human SNAP23. (DTNRDRIDIANARAKKLIDS) |
| 偶联标记 | Un-conjugated |
| 別名 | Vesicle-membrane fusion protein SNAP-23; SNAP23A; SNAP23B; HsT17016; Synaptosomal-associated protein 23; SNAP-23 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 23 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00161 Human Synaptosomal-associated protein 23 |
|---|---|
| 基因名称 | SNAP23 |
| 全名 | synaptosomal-associated protein, 23kDa |
| 背景介绍 | Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. [UniProt] |
| 细胞定位 | Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note=Mainly localized to the plasma membrane. [UniProt] |
| 预测分子量 | 23 kDa |
检测图片 (7) Click the Picture to Zoom In
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ARG41354 anti-SNAP23 antibody ICC image
Immunocytochemistry: K562 cells stained with ARG41354 anti-SNAP23 antibody.
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ARG41354 anti-SNAP23 antibody ICC/IF image
Immunofluorescence: T-47D cells were blocked with 10% goat serum and then stained with ARG41354 anti-SNAP23 antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG41354 anti-SNAP23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG41354 anti-SNAP23 antibody.
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ARG41354 anti-SNAP23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41354 anti-SNAP23 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41354 anti-SNAP23 antibody WB image
Western blot: Rat spleen, Rat testis, Rat ovary, HeLa, MCF7 and SKOV cell lysates stained with ARG41354 anti-SNAP23 antibody.
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ARG41354 anti-SNAP23 antibody FACS image
Flow Cytometry: HepG2 cells were blocked with 10% normal goat serum and then stained with ARG41354 anti-SNAP23 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41354 anti-SNAP23 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue stained with ARG41354 anti-SNAP23 antibody.
