ARG64061

anti-SNAIL antibody

anti-SNAIL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
nuts_pic
1
publication_link 参考文献1

概述

产品描述 Goat Polyclonal antibody recognizes SNAIL
反应物种 Hu, Rat
预测物种 Ms
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 SNAIL
抗原物种 Human
抗原 RKPSDPNRKPNY-C
偶联标记 Un-conjugated
別名 SNAH; SNAIL; SNA; dJ710H13.1; Protein sna; Protein snail homolog 1; Zinc finger protein SNAI1; SLUGH2; SNAIL1

应用说明

应用建议
应用 推荐稀释比
IHC-P5 µg/ml
WB0.1 - 1 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6615 Human SNAI1

Swiss-port # O95863 Human Zinc finger protein SNAI1

背景介绍 The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
预测分子量 29 kDa
翻译后修饰 Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.

检测图片 (5) Click the Picture to Zoom In

  • ARG64061 anti-SNAIL antibody WB image

    Western blot: 20 µg of HeLa and A549 cell lysates stained with ARG64061 anti-SNAIL antibody at 1:1000 dilution.

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded breast carcinoma tissue stained with ARG64061 anti-SNAIL antibody at 1:100 dilution. Antigen Retrieval: Boil tissue section in EDTA buffer (pH 9.0).

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64061 anti-SNAIL antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64061 anti-SNAIL antibody WB image

    Western blot: 35 µg of Rat kidney lysate (in RIPA buffer) stained with ARG64061 anti-SNAIL antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG64061 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded lung tissue from HGF overexpressing mouse stained with ARG64061 anti-SNAIL antibody at 1:500 dilution.

客户反馈

nuts_pic      Good

anti-SNAIL antibody

Application:WB

Sample:3T3 cell lysate

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Long non-coding RNA LINC01137 contributes to oral squamous cell carcinoma development and is negatively regulated by miR-22-3p.

WB / Human

Yong Du et al.
Cell Oncol (Dordr).,  (2021)

publication_link

 

hr_line