ARG56427

anti-SNAIL antibody

anti-SNAIL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes SNAIL
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 This antibody detects endogenous levels of SNAIL protein.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SNAIL
抗原物种 Human
抗原 Synthetic peptide around Ser246 of Human SNAIL.
偶联标记 Un-conjugated
全名 snail family zinc finger 1
別名 SNAH; SNAIL; SNA; dJ710H13.1; Protein sna; Protein snail homolog 1; Zinc finger protein SNAI1; SLUGH2; SNAIL1

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 29 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
纯度 > 95% (by SDS-PAGE).
缓冲液 PBS (pH 7.2) and 0.05% Sodium azide.
抗菌剂 0.05% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20613 Mouse SNAI1

GeneID: 6615 Human SNAI1

Swiss-port # O95863 Human Zinc finger protein SNAI1

Swiss-port # Q02085 Mouse Zinc finger protein SNAI1

基因名称 SNAI1
基因全名 snail family zinc finger 1
背景介绍 The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
生物功能 Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. [UniProt]
翻译后修饰 Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.

检测图片 (3) Click the Picture to Zoom In

  • ARG56427 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG56427 anti-SNAIL antibody at 1:100 dilution.

  • ARG56427 anti-SNAIL antibody WB image

    Western blot: 1) MCF-7, 2) NIH-3T3, and 3) Rat heart lysates stained with ARG56427 anti-SNAIL antibody at 1:500 dilution.

  • ARG56427 anti-SNAIL antibody WB image

    Western blot: 40 µg of 1) HCT116 whole cell lysate, 2) Mouse kidney, and 3) Rat kidney lysates stained with ARG56427 anti-SNAIL antibody at 1:500 dilution.